7 research outputs found
Quantifying protein diffusion and capture on filaments
The functional relevance of regulating proteins is often limited to specific
binding sites such as the ends of microtubules or actin-filaments. A
localization of proteins on these functional sites is of great importance. We
present a quantitative theory for a diffusion and capture process, where
proteins diffuse on a filament and stop diffusing when reaching the filament's
end. It is found that end-association after one-dimensional diffusion is the
main source for tip-localization of such proteins. As a consequence, diffusion
and capture is highly efficient in enhancing the reaction velocity of enzymatic
reactions, where proteins and filament ends are to each other as enzyme and
substrate. We show that the reaction velocity can effectively be described
within a Michaelis-Menten framework. Together one-dimensional diffusion and
capture beats the (three-dimensional) Smoluchowski diffusion limit for the rate
of protein association to filament ends.Comment: 13 pages, 7 figure
Two-Species Active Transport along Cylindrical Biofilaments is Limited by Emergent Topological Hindrance
Active motion of molecules along filamentous structures is a crucial feature of cell biology and is often modeled with the paradigmatic asymmetric simple exclusion process. Motivated by recent experimental studies that have addressed the stepping behavior of kinesins on microtubules, we investigate a lattice gas model for simultaneous transport of two species of active particles on a cylinder. The species are distinguished by their different gaits: While the first species moves straight ahead, the second follows a helical path. We show that the collective properties of such systems critically differ from those of one-species transport in a way that cannot be accounted for by standard models. This is most evident in a jamming transition far below full occupation, as well as in nonequilibrium pattern formation. The altered behavior arises because-unlike the case in single-species transport-any given position may be targeted by two particles from different directions at the same time. However, a particle can leave a given position only in one direction. This simple change in connectivity significantly amplifies the impact of steric interactions and thus becomes a key determinant of mixed species transport. We computationally characterize this type of hindrance and develop a comprehensive theory for collective two-species transport along a cylinder. Our observations show high robustness against model extensions that account for additional biomolecular features and demonstrate that even small fractions of a second species can significantly alter transport. This suggests that our analysis is also relevant in a biological context